Fig 2: PCAF, BRD4 determined by Western blot in anti-ISX immunoprecipitates of tumor tissues from patients with liver cancer

Fig 3: Downregulation of PCAF attenuates the levels of oxidative stress related markers in AngII-induced ECs and vascular tissue in mice. (A) Commercial kits to detect SOD, CAT and ROS activity in ECs (n = 5). ** p < 0.01 vs. the control group; ## p < 0.01 vs. AngII + Ad-GFP group. (B) Commercial kits to detect SOD, CAT and ROS activity in vascular tissue of mice (n = 5). * p < 0.05, ** p < 0.01 vs. the sham group; # p < 0.05, ## p < 0.01 vs. AngII group.

Fig 4: Nrf2/HO-1 mediates the anti-senescence effect of PCAF downregulation. (A) ECs cells were lysed for immunoprecipitation (IP) assays, and PCAF or Nrf2 were detected by Western blot in the precipitates pulled down by antibody against PCAF or normal IgG. Input indicates the cellular lysate before adding antibodies (n = 3). (B,C) The total expression of Nrf2 and the expression of Nrf2 in nucleus, detected by western blot (n = 6). (D) Immunofluorescence to detect the distribution of Nrf2 (green); arrows represent nuclear translocation of Nrf2; the scale bar is 50 µm, 400× (n = 3). (E,F) HO-1 and NQO-1 protein expression (n = 6). ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. AngII + Ad-GFP group.

Fig 5: AFAP1-AS1 promotes YAP mRNA stability via KAT2B. (A, B) AFAP1-AS1 depletion inhibited YAP protein (A) and mRNA (B) expression. (C–G) Effects of KAT2B overexpression on AFAP1-AS1 depletion: inhibited YAP protein expression (C), YAP mRNA expression (D), cellular proliferation (E), colony formation (F), and YAP mRNA stability (G). (H) Schematics of full-length AFAP1-AS1 and AFAP1-AS1 deletion mutants at the nitrogen (N) or carbon (C) protein sites. (I) Effects of AFAP1-AS1 plasmids in (H) overexpressing HNE-1 cells. (J–M) Effects of AFAP1-AS1 plasmid in (H) overexpressing cells on YAP mRNA expression (J), YAP protein expression (K), cellular proliferation (L), and colony formation (M). Error bars represent the standard deviation. *P < 0.05. **P < 0.01. ***P < 0.001. The data represent three independent experiments.